Chandipura virus (CHPV), initially thought to be an orphan virus, was later reported to cause sporadic cases of fever with arthralgia, Reye’s syndrome, and. Chandipura virus (CHPV; genus Vesiculovirus, family Rhabdoviridae) is an emerging tropical pathogen with a case fatality rate of 55 to 75% that predominantly. Chandipura virus: A virus that causes fever, symptoms similar to those of flu, and acute encephalitis (inflammation of the brain). Chandipura virus was first.
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Chandipura virus CHPVan arbovirus belonging to genus Vesiculovirus in the family Rhabdoviridae has gained global attention as an encephalitis causing virus after the outbreaks in central India 12345. The significance of Chandipura virus as a human pathogen is unresolved due to doubts over its role in the and outbreaks.
However, control of vectors in endemic areas is difficult as they breed in damp places inside crevices of stone used for construction where insecticide spraying is generally not possible. RNA viruses are known for mutation as it provides evolutionary advantages over their host organisms in survival.
Rhabdomyosarcoma and Phlebotomus papatasi cell lines as well as embryonated chicken eggs have been found useful in virus isolation firus propagation. Analysis of 12 pairs of putative protein interactions among N protein fragments and CHPV proteins revealed a total of 9 positive interactions.
Immunofluorescent antibody technique IFA and virus isolation using cell lines: Even a two dose vaccine yielded per cent protection in seroconverted mice.
Guravand V. Blood brain barrier breakage and virus replication in central nervous system was observed when inoculated through IC or intravenous routes Reprinted with permission from: Spraying of insecticides could bring down the population drastically.
Chandipura Virus: an emerging tropical pathogen.
Isolation of Chandipura virus Vesiculovirus: After its discovery in and subsequent isolation from sandflies, no cases of human involvement or any outbreak of public health importance were reported from the area or elsewhere for approximately two decades. The outbreaks in Central India witnessed case fatality rates ranging from per cent in Andhra Pradesh and Gujarat with typical encephalitic symptoms.
Both these phenomena cohesively explain the neuropathogenesis and neurodegeneration following CHPV infection. Recombinant glycoprotein based vaccine for Chandipura virus infection.
Chandipura virus CHPV is a recently recognized emerging human pathogen [ 1 — 3 ] of the genus Vesiculovirus and family Rhabdoviridae [ 4 ]. Experimental studies with Phlebotomus papatasi showed their potential not only to replicate the virus but also to transmit the virus through vertical, venereal and horizontal routes 23 Serological studies also demonstrated presence of N-antibodies in pigs and other domestic animals in the affected areas 1.
Chandipura Virus: an emerging tropical pathogen.
Significant contributions have been made in the development of diagnostics and prophylactics, vaccines and antivirals. Author information Article notes Copyright and License information Disclaimer. In the field of antivirals, siRNAs have been found to be promising as inhibition of virus replication was observed both in vitro and in vivo The recombinants were confirmed by restriction enzyme digestion.
Am J Trop Med Hyg ; Retrospective studies with human serum samples collected from to from different parts of the country showed prevalence of N-antibodies in humans and domestic animals across the country except in Kashmir and Arunachal Pradesh J Virol ; Potential of CHPV to cause mortality in humans was detected when the virus was held solely responsible for the death of an 11 yr old child in Raipur district, Madhya Pradesh now in Chhattisgarh State.
Clearance of virus infection from the CNS. Full-length CHPV viral genes had been previously transformed in corresponding yeast strains and checked for autoactivation viruus earlier studies by the authors [ 14 ].
Antivirals In the field of antivirals, siRNAs have been found to be promising as inhibition of virus replication was observed both in vitro and in vivo Our data corroborates well with the previously identified interacting regions involved in NN and NP interactions for both CHPV [ 1213 ] and VSV [ 30 chandilura, thus validating our approach of interaction analysis.
Chandipura virus – Wikipedia
Vector control CHPV is transmitted by Phlebotomine sandflies as evidenced by repeated isolations and their ability to transmit the virus viruz transovarial and venereal routes 2324 The vaccine produced per cent seroconversion after the third dose in mice Fig. Chandipura virus induces neuronal death through Fas-mediated extrinsic apoptotic pathway. The interaction of monomeric N protein with P maintains it in the encapsidation competent soluble active form [ 78 ].
A combination of the former with commercially available DPT vaccine induced high antibody titres for both the vaccines demonstrating its potential application in the national programme of vaccination.