Seroprevalencia de la cisticercosis porcina en tres caseríos de la provincia de Andahuaylas, Apurímac. Article (PDF Available) in Revista de Investigaciones. AYVAR P., Viterbo et al. Seroprevalencia de la cisticercosis porcina en tres caseríos de la provincia de Andahuaylas, Apurímac. Rev. investig. vet. Perú [ online]. Publisher: Efecto in vitro de la vacuna S3Pvac contra cisticercosis en para prevenir la cisticercosis porcina e interrumpir la transmision.
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Palabras clave Key words: Cisticercosis porcina swine cysticercosis ; inmunoblot immunoblot ; Taenia crassiceps.
Its economic and sanitary effects n society are more important in developing countries in Asia, Africa and Latin America, where this disease should be considered a public health priority. Swine infected with T.
Cisticercossi high frecuency of human cysticercosis and the severity of the neurological manifestations occurring in this condition, with high morbidity and lethality, indicate the importance of the taeniasis-cysticercosis complex, justifying the application of effective measures of diagnosis and control of these illnesses in man and swine.
ELISA and immunoblot are particularly important among the immunological tests used for the diagnosis of swine cysticercosis Gonzalez et al. Investigating the prevalence of swine oprcina in an endemic area with four different tests, Gonzalez et.
Due to the difficulty in obtaining larval antigens from T. Because of the incipient application of the immunoblot in the diagnosis of swine cysticercosis compared to human cysticercosis, the objective of the present study was to evaluate an immunoblot methodology using total antigen of T. Based on anatomopathological swine examination, we analyzed by immunoblot 13 sera from swine with cysticercosis, 30 sera from negative controls and 35 sera from swine with no cysticercosis but with other diseases, i.
Immunoblot Crude antigen of T.
The total cysts were dehydrated by lyophilization and then pulverized in a mortar. Saline was added to the material cissticercosis homogenization with a Potter type blender in an ice bath. Strips of 3 to 4 mm width were obtained from the membrane blots and washed three times in saline solution 0.
Serum samples diluted in the 1: After six washes of 5 minutes each, the strips were incubated for one hour with peroxidase-labeled anti-swine IgG conjugate A The reactive peptides were revealed with a chromogenic solution 5 mg diaminobenzidine, 1. The reactive bands were analyzed by cisticercsois with a GS densitometer and Rf and Disticercosis were calculated with the aid of the Molecular Analyst Program, version 1. The immunoblot was standardized by checkerboard titration using a positive and negative control serum.
The best block was then confirmed by testing three positive high, medium and low reactivity and three negative control sera.
PAHO/WHO IRIS | Investigacion de la cisticercosis porcina en San Luis Potosi, Mexico –
Analysis of the reactive peptides that discriminate between swine with cysticercosis and swine without cysticercosis. The location of the reactive bands on the nitrocellulose strip, as well as their physical appearance, particularly the color intensity, were considered in the interpretation of the reactivity and enumeration of the bands Larralde, et al. The frequency of the reactive bands, the sensitivity, specificity, and positive and negative predictive values were calculated for each peptide in order to establish the criterion for serum differentiation as positive or negative for swine cysticercosis, or beter, for the definition of the specific peptides and for the establishment of the positive and negative criteria.
After the criteria were established, the performance of the immunoblot was evaluated on the basis of the results for each group of swine. RESULTS The results of checkerborad titration showed that the best dilutions for the differentiation between positive and negative sera were 1: Despite this high sensitivity, the two peptides showed 7. The analysis of immunoblot data for all groups showed reactivity with 29 peptides ranging from kD to 14 kD.
This analysis was used to determine three criteria to differentiate sera of swine with cysticercosis from sera of swine that did not carry the disease Table I. Frequency of specific peptides and their performance rates in the immunoblot with Taenia crassiceps antigens, according to cysticercosis positive and non-cysticercosis negative and other diseases groups.
Thus, the results shown in Table I led to the identification of nine specific peptides: Reactivity with two or more specific peptides was considered positive. Four positive control sera The porcija number of bands that reacted with positive serum This serum corresponded to 2. One negative control serum 3. Reactivity of control sera in the immunoblot with Taenia crassiceps antigen other diseases: H hydatidosis, A ascariadiosis, M macracanthorhincosis, P pneumonia.
Several factors interfere with the performance of the immunoblot methodology, especially the need for trained personnel. The adequate and correct identification of reactive bands, as well as their enumeration depend on the researcher’s experience, with special attention to their physical appearance and location on the nitrocellulose strips. Also, the mean value found in the analysis of the positive control samples should be considered, as well as the frequency of each reactive band for cysticercosis.
The efficiency of the immunoblot can also vary according to antigen lot, electrophoretic separation and transfer Gershoni and Palade, ; Tsang et. The low sensitivity of some specific peptides kD and 14 kD seems to have been influenced by deficiencies in the transfer process of some of them to the nitrocellulose strips, which impaired some reactions with the antibodies of the positive control serum or rendered others too weak for visualization.
This effect was also caused by the individual variation of the immune response, since pircina lot of swine positive controls was quite heterogeneous Tsang et. In a study on the immunologic response of pigs with cysticercosis from an endemic area and from a non-endemic area, Gonzalez et.
The reactivity of the immunoblot with positive sera of from the non-endemic area was less intense than that observed in the sera from highly infected animals. This difference was porcinaa previously to factors discussed by Tsang et. It should be pointed out that the average occurrence of specific peptides was 0.
According to Tsang et. All the swine experimentally infected with eggs of T.
Investigacion de la cisticercosis porcina en San Luis Potosi, Mexico
Of course, the criteria for defining positivity can vary according to the purpose of the research, i. Peptides similar to these defined as specific in our investigation were considered important for the diagnosis of human cysticercosis using T.
These glycopeptides cisyicercosis also reactive with sera from swine three weeks after experimental infection Tsang et al.
Other authors have been studying T.
The kD peptide identified in the present study showed one of the lowest specificity rates In the present study, the serum from one swine with discrete lesions of predominantly cerebral location showed a weak reaction, in agreement with data reported by Pathak et. We did not find any other published paper reporting the use of T. The immunoblot performed here showed Without affecting the good performance of the immunoblot, hydatidosis was the only disease that revealed antibodies with the potential capacity of reacting with specific peptides e kD poorcina the antigen used.
Similarly to us, Montenegro et al. Considering the large number of specific peptides identified in the T-cra antigen, our results indicate the feasibility of its use, overcoming the difficulties in locating sources of T.
The use of T. The high cisticercpsis of swine cysticercosis and of human cysticercosis and teniasis expected in certain countries justifies the application of efficient diagnostic methods that will permit effective measures of disease control both in terms of public health and animal health.
Experimental Taenia solium cysticercosis in pigs: Immunodiagnosis of swine cysticercosis by indirect ELISA employing the heterologous antigen from Taenia crassiceps metacestode.
Specific Taenia crassiceps and taenia solium antigenic peptides for neurocysticercosis immunodiagnosis using serum samples. Immunodiagnosis of human cysticercosis Taenia solium: Immunotherapy for porcine cysticercosis: Prevalence and comparison of serologic assays, necropsy, and tongue examination for the diagnosis of porcine cysticercosis in Peru. Use of sentinel pigs to monitor environmental Taenia solium contamination.
The Cysticercosis Working Group in Peru. Persistence of passively transferred antibodies cisticrecosis porcine Taenia solium cystercosis. Demonstration of species-specific and cross-reactive components of T.
Evaluation of the characterization of purified antigenic fraction-II of Cysticercus cellulosae by enzymelinked immunosorbent assay for the diagnosis of neurocysticercosis before and after treatment.
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The diagnostic importance of species specific and cross-reactive components of Taenia soliumEquinococcus granulosusand Hymenolepis nana. Effective immune protection of pigs against cysticercosis. ELISA test for the diagnosis of cysticercosis in pig using antigens of Taenia solium and Taenia crassiceps cysticerci. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Enzyme-linked immunoelectrotransfer blot tecnhiques EITB for studying the specificities of antigens and antibodies separated by gel electrophoresis.
An Enzyme-linked immunoelectrotransfer blot assay and glycoproteins antigens for diagnosing human cysticercosis Taenia solium. Immunoblot with cerebrospinal fluid from patients with neurocysticercosis using antigen from cysticerci of Taenia solium and Taenia crassiceps. Immunoblot analysis using antigen from Taenia crassiceps cysticerci in the diagnosis of swine cysticercosis. Casilla Santiago, Chile halcaino uchile.