Evolution and Functional Diversification of Fructose Bisphosphate Aldolase have indicated particularly diverse origins of their overall gene repertoire. fructose 1,6-bisphosphate aldolases (FBAs) illustrate the influence on. Fructose-1, 6-bisphosphate aldolase (FBA) is a key plant enzyme that is Gene Evolution, and Expression Analysis of the Fructose-1, 6-bisphosphate cloning , and evolutionary history of the chloroplast and cytosolic class I aldolases of the . Fructose-bisphosphate aldolase (EC ), often just aldolase, is an enzyme catalyzing a . “Fructose-bisphosphate aldolases: an evolutionary history”.
|Published (Last):||10 January 2017|
|PDF File Size:||9.65 Mb|
|ePub File Size:||15.38 Mb|
|Price:||Free* [*Free Regsitration Required]|
The aldolase used in gluconeogenesis and glycolysis is a cytoplasmic protein. Each member of the FBA gene family might thus have different biochemical activity. On the other hand, all class I CpFBA genes were apparently sensitive to multiple stresses, as indicated by a repression in expression.
Protein domains Lyases Moonlighting proteins. Various patterns of exon-intron architecture were identified. Promoters are shown as light-blue rectangles. Both classes have been found widely in other eukaryotes and in bacteria.
We will be provided with an authorization token please note: Gene duplication not only expands genome content but also diversifies gene function to ensure optimal adaptability and evolution of plants Xu et al. In the present study, we investigated the responses of wheat FBA genes family to salt, drought, ABA, heat, and low temperature.
The evolutionary study on plant FBA genes is useful in investigating the origin of chloroplast and photosynthetic genes. With few exceptions only class I proteins have been found in animalsplantsand green algae. One key factor in the success of wheat as a global food crop is its adaptability to a wide variety of climatic conditions. Gluconeogenesis and the Calvin cyclewhich are anabolic pathwaysuse the reverse reaction. Predicted structures of TaFBA protein monomers and homo-oligomers.
Total RNA was isolated from etiolated two-leaf seedling at 0, 1, 4, and 8 h after exposure to light.
Fructose-bisphosphate aldolase class-I active site (IPR) < InterPro < EMBL-EBI
Pairwise alignments of FBA genes of wheat and wheat relatives. Treatment of the wheat seedlings was performed as described Zeng et al. Putative cis-acting regulatory DNA elements in the promoter region 1. Short interspersed nuclear element SINEs are shown as green rectangles. The thermal cycle protocol was set up as follows: Gene expression analysis based on microarray and transcriptome databases suggested the distinct role of TaFBAs fructose-bisphpsphate different tissues and developmental stages.
Glycolysisa catabolic pathwayuses the forward reaction. As a single group of functionally related enzymes spanning two distinct gene families, fructose 1,6-bisphosphate aldolases FBAs illustrate the influence on core biochemical pathways of specific evolutionary associations among diatoms and other chromalveolates with various plastid-bearing and bacterial endosymbionts.
Fructose-bisphosphate aldolase – Wikipedia
Terminal inverted repeat TIRs are shown as red rectangles. The use, distribution or reproduction in other forums is permitted, provided the original author s or licensor are credited and that the original publication in this journal is cited, evolutionagy accordance with accepted academic practice. Sign In or Create an Account. No use, distribution or reproduction is permitted which does not comply with these terms.
We investigated the molecular characteristics, chromosomal location, gene evolution, protein structures, and expression patterns of these genes. The molecular evolutionayr of TaFBA genes and the prediction of chromosomal and subcellular location. Gold circle represents Euryarchaeota.
In glycolysis fructose 1,6-bisphosphate is made into glyceraldehydephosphate and dihydroxyacetone phosphate through the use of aldolase.
The Escherichia coli galactitol operon protein, gatY, and N-acetyl galactosamine operon protein, agaY, which are tagatose-bisphosphate aldolaseare homologs of class II fructose-bisphosphate aldolase. Characterization, cloning, and evolutionary history of the chloroplast and cytosolic class I aldolases of the red alga Galdieria sulphuraria.
The molecular characteristics of the TaFBA genes and their subcellular location histiry listed in Table 1. The word aldolase also refers, more generally, to an enzyme that performs an aldol reaction creating an aldol or its reverse cleaving an aldolsuch as Sialic acid aldolasewhich qldolases sialic acid. The etiolated wheat seedlings were cultured under continuous light, whereas the normal-cultured seedlings were placed in the dark after being exposure to light for 24 h.
Additionally, tyrosine residues are crucial to this mechanism in acting as stabilizing hydrogen acceptors. Combined with the results of Genevestigator, we analyzed the spatiotemporal expression profiles of the members of the TaFBA gene family Figure 6.